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ALMOST 20 YEARS EXPERIENCE IN CREATING INNOVATIVE SCIENTIFIC SOLUTIONS

Introduction

In monoclonal antibody (mAb) manufacturing, accurate antibody titre determination and monitoring are crucial for clonal selection, optimisation of expression rates, process control and scale-up. For accurate antibody titre quantification, Protein-A Affinity HPLC offers excellent reproducibility, robustness and precision over ELISA-based assays.

The SOLAD™ Protein-A is a novel affinity HPLC column, based on the next-gen SOLAD™ platform technology, for quick separation and robust quantification of various antibodies. It provides rapid, accurate titre analysis with linearity across a wide concentration range, without any carryover.

 

SEM of SOLAD Particles (2500x magnified)

Highlights

❖ Wide dynamic range

❖ Fast Analysis

❖ High sensitivity & specificity for mAbs

❖ Long lifetime

 

SOLAD Protein-A Particles zoomed
SEM of SOLAD Particles (10000x magnified)

Column Technology

SOLAD™ is a proprietary product range of highly monodisperse, spherical, durable and reproducible non-porous silica particles manufactured by Glantreo. For protein and antibody analysis, SOLAD™ offers excellent mechanical and chemical stability, with minimal fouling and non-specific adsorption, compared to porous silica and polymeric particles.

The SOLAD™ Protein-A column combines a 10µm sized SOLAD particle with recombinant Protein A ligand through proprietary cutting-edge bonding technology. The result being a hydrophilic resin surface with a high pH stability and a rugged and reproducible column.  The column offers superior efficiency and resolution, without analyte carryover.

The SOLAD™ Protein-A column can accurately measure mAb titres at various phases of development due to its high loading capacity and excellent repeatability. This column is also suitable for small-scale purification of mAbs for initial characterisation due to its reduced Protein A leaching. The column is available in PEEK and Bioinert Stainless Steel column hardware in various dimensions.

SOLAD Protein A Columns

SOLAD Protein-A Column PDS

A pdf version is available for Print/Download

Want to know more about our SOLAD range of Columns?

Go to the Product Page.

Technical Specifications and Applications of SOLAD Protein-A Column

Rapid separation of IgG from Impurities (Fig. 1)

Chromatographic Conditions
Column: 4mm x 50 mm PEEK, Binding buffer (A): 50 mM Sodium Phosphate pH 7.4, Elution buffer (B): 50 mM Sodium Phosphate pH 2.5, Gradient: 0%B: 0-0.5 min, 100%B: 0.5-1.1 min, 0%B: 1.1-4 min, Flow rate: 2 mL/min, Detection: UV @ 280 nm, Temperature: 25 C, Sample: 20 µL cell culture supernatant containing mAb
Rapid separation of IgG from impurities

Figure 1 depicts a 20 μL injection of mAb feedstock onto the SOLADTM Protein A column. The proprietary column technology yields sharp, concentrated peaks and fast, efficient elution.

Wide Dynamic Range (Fig. 2)

 

Wide Dynamic Range

As can be seen in Figure 2, SOLADTM Protein-A delivers accurate titre measurement with linearity, with a dynamic loading capacity in excess of 300 µg.

Flexible Flow Rates (Fig. 3)

Flexible Range of Flow Rates

The SOLADTM Protein-A column can be used from flow rates upto 2.5 mL/min, as seen in Figure 3. This allows fast separation of antibodies without loss of efficiency.

SOLAD Protein-A Column PDS

A pdf version is available for Print/Download

Want to know more about our SOLAD range of Columns?

Go to the Product Page.